Consulta: creatorFacets:"Zhou,Bin"
Registros recuperados: 2
Data/hora: 18/05/2024 05:27:20

Anti-Trimeresurus albolabris venom IgY antibodies: preparation, purification and neutralization efficacy
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Duan,Hai-long; He,Qi-yi; Zhou,Bin; Wang,Wen-wen; Li,Bo; Zhang,Ying-zheng; Deng,Qiu-ping; Zhang,Ying-feng; Yu,Xiao-dong.
Abstract  Background  Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found in the country, the white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, the present study was conducted aiming to develop an alternative antivenom antibody (immunoglobulin Y - IgY) from leghorn chickens.  Methods  IgY in egg yolk from white leghorn chicken previously injected with T. albolabris venom was extracted by water, precipitated by ammonium sulfate and purified by affinity chromatographic system. IgY was identified by SDS-PAGE, ELISA and Western blot, and its neutralizing assay was conducted on mice.  Results  Chickens injected multiple times...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IgY; Egg yolk; Snake venom; Trimeresurus albolabris; LD50.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100316

Preparation and neutralization efficacy of IgY antibodies raised against Deinagkistrodon acutus venom
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Liu,Jinhua; He,Qiyi; Wang,Wenwen; Zhou,Bin; Li,Bo; Zhang,Yingfeng; Luo,Cong; Chen,Diancheng; Tang,Jia; Yu,Xiaodong.
Abstract  Background  The five-paced pit viper (Deinagkistrodon acutus), endemic to China and northern Vietnam, is responsible for most snakebites in the Chinese territory. Antivenom produced from horses is the main treatment for snakebites, but it may cause numerous clinical side effects and have other disadvantages involved in their production such as the welfare of animals. The present study was conducted aiming to develop an alternative antibody (IgY) from the egg yolk of leghorn chickens immunized with snake venom.  Methods  IgY from the egg yolk of white leghorn chickens previously immunized intramuscularly with D. acutus venom was extracted by water, precipitated by ammonium sulfate and purified by affinity chromatographic system. IgY was identified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IgY antibody; Egg yolk; Snake venom; Snakebite; Deinagkistrodon acutus; Venom neutralization.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100310